What is the maximum sterilization temperature for removable, autoclavable pump heads?
For Minifors, Labfors and Multifors pump heads, sterilization at 134°C (sometimes used for deocontamination) will result in damage to a key component of the pump head. Temperatures up to 125°C should be fine i.e. normal sterilization temperature. There are two possible work-around solutions:
- Remove the tubing from the vessel inlet pipes after emptying and clamping off the lines. Sterilize the bottles, pump heads and tubing at e.g. 121°C.
- Snap open the pump head and remove the tubing to allow the whole line to be sterilized with the vessel and bottle but without the pump head. This makes a little more work when preparing the system but allows the higher temperatures wjhen this is a necessity.
What shall I take into account when working with heat labile media and/or medium constituents?
It is possible to autoclave the vessel without media or sensitive constituents. Autoclave the vessel with enough water to cover probe tips. Drain the vessel via the harvest pipe and add media after the vessel cools. Be sure to do this in a sterile environment such as a laminar flow hood.