BIOEXCEL M

Autoclavable Bioreactor

Lab Scale FERMENTOR for Microorganism cultivation


BIOEXCEL M

Lab Scale FERMENTATION System

What is Fermentation?

Fermentor cultivates microorganisms, producing food, biomedicine (vaccine, antibiotics), vitamin, enzyme, and insecticides. One of the most well-known applications of micro-organisms in industry is fermentation, where carbohydrates are converted into an acid or an alcohol.
Fermentation is mostly extracellular activity and is brought with the help of enzymes released by the microorganisms. Microorganisms, such as fungi, algae, bacteria, protozoa, are increasingly important to industry, where they are used in a huge variety of processes ranging from food production to water treatment.

BIOEXCEL M Fermentor!

Fermentor can be categorized according to shape, uses, and cultivation method. Usually, fermentor with volume from 1L to 19L is characterized as Lab Scale Fermentor. BIOEXCEL M is a sensational concept of fermentation based on interactive control system. The BIOEXCEL M system is a lab scale fermenter and bioreactor designed for multipurpose fermentation and cell culture. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. BIOEXCEL M controller offers various bioreactor functions. Hi-res color touch screen, easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted.


The BIOEXCEL M’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement: 180mm(W), 15kg, slim design.  The BIOEXCEL M’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication.  Variable vessels are available for bacteria, yeast or fungi, and with optional accessories can be adapted for mammalian, animal, insect or plant cell. Vessels used for BIOEXCEL M are basically for microorganisms such as bacteria, yeast or fungi. However, This can be also used for mammalian, animal, and insect or plant cell culture with optional device like 4-gas mixer.  BIOEXCEL M is basically able to do batch, fed-batch, and continuous culture mode for microorganism fermentation. also, these three culture modes can be used for animal cell, plant cell, and microalgae, but please note that all functions which can satisfy customer’s every single demand cannot be offered. Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps(acid, base, antifoam and feed), DO, pH, Temp, Foam, sensors are available. The graphic user interface program tracks the status of the fermentation system in real time. You can use 4 peristaltic pumps for feeding as well as external pumps.


Application of fermentation in Bio-Industry

Foods that are produced by using these methods include cheese, yoghurt, butter, beer, wine and bread. Fermentation is mostly extracellular activity and is brought with the help of enzymes released by the microorganisms. The end products or the various intermediate products (primary and secondary metabolites) of the fermentation activities of many microorganisms are highly useful. Hence, microorganisms have been commercially extended by fermentation industries. One of the most important features of fermentation is that micro-organisms can be grown in a physiological steady state. In steady state, growth occurs at a constant rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition environmental conditions can be controlled by the experimenter. Micro-organisms grown in chemostats naturally strive to steady state: if a low amount of cells are present in the bioreactor, the cells can grow at growth rates higher than the dilution rate, as growth isn’t limited by the addition of the limiting nutrient. The limiting nutrient is a nutrient essential for growth, present in the media at a limiting concentration. Micro-organisms grown in chemostats naturally strive to steady state: if a low amount of cells are present in the bioreactor, the cells can grow at growth rates higher than the dilution rate, as growth isn’t limited by the addition of the limiting nutrient. The limiting nutrient is a nutrient essential for growth, present in the media at a limiting concentration

BIOEXCEL M Fermentor Specifications

Summary of features and strength

The BIOEXCEL M system is a lab scale fermenter and bioreactor designed for multipurpose fermentation and cell culture. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. Usually, fermentor with volume from 1L to 19L is characterized as Lab Scale Fermentor.
The BIOEXCEL M’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication. The BIOEXCEL M’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement and slim design.
Variable vessels are available for bacteria, yeast or fungi, and with optional accessories can be adapted for mammalian, animal, insect or plant cell. Vessels used for BIOEXCEL M are basically for microorganisms such as bacteria, yeast or fungi. However, This can be also used for mammalian, animal, and insect or plant cell culture with optional device like 4-gas mixer. Additional exterior 4 channel gas mixer can be used.
Easy to do Batch, Fed-Batch, Continuous culture fermentation, and it enables to cultivate micro-organism by accurate adjustment of temperature, agitation speed, pH, DO, and foams (which generate during the fermentation) via microprocessor and PC. Also, these three culture modes can be used for animal cell, plant cell, and microalgae, but please note that all functions which can satisfy customer’s every single demand cannot be offered.
Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps(acid, base, antifoam and feed), DO, pH, Temp, Foam, sensors are available.
BIOEXCEL M is a sensational concept of fermentation based on interactive control system. Color touch screen and easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted. Core competence of BIOEXCEL M is providing perfect control reflecting the newest IT trend and changeover ideas for users to develop their own methods.
BIOEXCEL M Controller PID controls pH, Temperature, Agitation speed, and DO by Cascade method. Cascade method is adopted for agitation speed or mixing gas. Fed-batch culture are available based on pH, DO. This fed-batch culture method maintains vessel inside condition based on constant level of pH, DO. Continuous culture can be also used as continuous feeding is available in the system.
BIOEXCEL M Controller supports pH, Do, temperature and foam sensor. It also includes 4 peristaltic pumps for Acid, Base, Antifoam, and feeding for fermentor.
Generally, motor for agitation is installed in top plate of vessel, and simple and durable agitation motor is used. In some case, Bottom magnetic Drive system can be applied. Various size options are available for Single vessel, Double wall vessel, Bowl vessel, Single round bottom vessel, and Small volume vessel. For cell culture such as animal or plant cell, servo motor can be used for agitation in very slow speed with accurate control of speed.
Digital PID control algorithm can control the temperature very fast, and stable temperature is maintained. pH value is calibrated in consideration of temperature, and can be controlled by feeding acid and base during fermentation. DO value is adjusted to measure accurate value according to temperature and salinity.

Features
  • BIOTRON BIOEXCEL M Fermentation system is a lab scale bioreactor designed for multipurpose cell culture.
  • The size of vessel can be decided upon the working volumes of real media and we provide interchangeable vessels that are convenient to scale up vessel among the various size of vessels.
  • Qualified for dense fermentation : Batch fermentation, DO-stat and pH-stat fed-batch, continuous culture, etc. with microprocessors controlling Temp, pH, DO, agitation and peristaltic pumping for various fermentation procedures on the control panel.
  • BIOEXCEL M Control system shows the measured values of Temperature, pH, DO, Foam, Motor speed and the status of peristaltic pumps at the same time.
  • Monitor and manage data from anywhere! Save and manage data on a PC by simply connecting the controller to a PC.
  • The graphic user interface program tracks the status of the fermentation system in real time.
  • You can use 4 peristaltic pumps for feeding as well as external pumps.
  • All measured data and control parameters are transmitted to a PC and wireless communication protocol.
  • The BIOEXCEL M bioreactor’s 7-inch wide control panel screen, Built-in 7” color touch screen; easy-to-learn controller with intelligent GUI.
  • Sensor port and communication port are located in the front for the user’s convenience and allows side by side placement: 180mm (W), 15kg, and slim design. Side-by-side location of vessel and controller: 4 peristaltic pump and pH, DO, foam, temperature sensor port.
  • Easy to calibrate temperature, pH, DO, foam sensors when using graphic calibration mode.
  • The motor speed can be controlled by the DO value in DO mode to control the dissolved oxygen level. Agitation motor speed controlled by Dissolved oxygen rate in the DO mode automatically.
  • User can control fed batch by DO mode or accelerate motor speed Fed batch function and motor speed controls can be controlled by DO control mode.
  • User can choose feeding time so as to have stability and controllability.
  • The pH and DO value precisely with the temperature, and the value of dead-band for the peristaltic pump control can be adjusted. pH, DO offset: A operating instant of pH can be setting up by user, which resolve any control problems are occurred. User can choose feeding time so as to have stability and controllability. DO value from Dead band lead to certain point of feeding.
  • Intelligent self-diagnostic system to maintain fermentation process from start to end: Diagnostic, Setup, Dead Band, Offset, Warning, Feeding condition.
  • Easy control of external device by fermentation controller. And PC remote controls via data logging system.
  • Connected to glucose analyzer, Collect signal and send it to computer system by MFC device. (Option)
  • Pure glass touchscreens for superior image clarity durable, scratch resistant glass surface Finger, gloved hand, soft stylus activation Fast sensitive touch response Flat, spherical, cylindrical touchscreen,
  • Trend Data Viewer: BIOEXCEL M controller shows parameter data such as pH, DO, Temperature, Agitation, Pump status during fermentation process in real time. For PC, this data is displayed as graph and past graph is accumulated and showed on the monitor.
  • Graph check and PID Value adjustment for temperature Test. By adjusting PID according to install environment, accurate temperature control is available. Temperature control test function shows actual temperature control situation on the graph.

BIOEXCEL M (RED) with single Vessel and 4 Gas Mixer 1

BIOEXCEL M (White) with single Vessel and 4 Gas Mixer 1
Specifications
Agitation Drive Top drive motor (mechanical drive)
Range 50~1,200 rpm
Sensor Magnetic hall sensor
Impellers 2-Rushton turbine impeller, 1-foam breaker
Indication Monolitic LCD display
Temperature Range From 3℃ above tde cooling water temperature to 80 (accuracy ±0.1℃)
Control Digital temperature controller
Sensor RTD(Pt-100)sensor
Aeration 2 Gas Mode Air, O2
4 Gas Mode Air, O2, CO2, N2,(in cell culture, in plant cell culture)
Air Flow, Electronic TMM or TMC
Sparger Ring sparger
Inlet Filter O.2㎛ air filter cartridge
Exhaust System Filter O.2㎛ air filter cartridge
Condenser SUS316
pH Setpoint / Display Range 2.0 ~ 12.0
Control Modes PID maintain witd 0.01 pH of setpoint
Electrode Mettler Toledo or Hamilton electrode
DO Setpoint Range 0 ~ 200% ±0.1
Display Range 0 ~ 200% ±0.1
Control Modes PID
Electrode Mettler Toledo or Hamilton electrode
Control Cabinet Dmension 250 D x 350 W x 300 H(mm)
Power 220V, 50/60Hz
Peristaltic Pumps Control 4 pumps each assignable to a wide variety of function for control of acid, base, foam and feed
Recording Output pH, DO, Foam, Temperature,Agitation, RS-232C
Heating Plate Electric Heater 400 watt
Dimension 250 D x 300 W x 100 H(mm)
Control PID

* Please note that technical specifications are subjected to be changed.

Specifications of BIOEXCEL M Bioreactor Controller
Main Controller
  • CPU                              : 32Bit ARM9 CPU, 266MHZ
  • Main Memory            : 64MB SD-RAM
  • Secondary Memory   : 64MB Flash Memory, 128MB Flash Memory for Data Logging
  • Watch Dog Timer      : Recovery when the system is down suddenly.
  • Real Time OS             : Developed Real Time Operating System
  • Real Time Clock
Measurement System
  • pH/DO/Temp/Pressure/Balance/Foam measurement analog board only communicate with main controller
  • Separated board Box
  • RS422A communication method
  • All sensor with separated power
  • pH Measure and Control System
  • Measurement and display range : 0 ~ 14.00
  • Control        : PID
  • Control output : Peristaltic pump signal and Gas-Mixer signal
  • maintained within 0.01 pH of set point Selectable dead band of 0.01pH also available via keypad
  • Three-point auto calibration
  • Buffer recognition at pH 4.00, 7.00 and 10.00 buffer values
  • Automatic temperature compensation for greater accuracy at varying temperatures.
  • Simultaneously display pH(or mV) and temperature readings.
  • mV measurement display allows checking electrode performance.
DO Measure and Control System.
  • Measurement and Display range : 0.0 – 20.0ppm or 0.0 – 100.0%
  • Temperature compensation : PT100 sensor
  • Measurement range : 0~150 ℃
  • Read dissolved oxygen in mg/L, ppm, or % saturation with simultaneous temperature display
  • Automatic temperature compensation
  • Simultaneously display mV and temperature readings.
  • mV measurement display allows to check electrode performance.
Temperature Measure and control System
  • Measuring range  : 0 – 150℃, Sterilizable
  • Accuracy         : f.s. ± 0.3 %
  • Sensor           : PT100 ohm double ceramic sensor with ready mounted cable
  • Measuring principle : Linear resistance type
Foam / LEVEL
  • Conductivity method
  • Sensitivity adjusting kit
Motor Control Board
  • Motor       : DC Motor/AC Motor
  • Diagnostics : Inverter selection

BIOEXCEL M Red

BIOEXCEL M White
Summary of BIOEXCEL M Bioreactor Program

Measuring : Set value and Present value

  • Sensor          : pH, DO, Temperature
  • Agitation      : RPM
  • Foam sensitive
Time
  • Start Time              : Start of Fermentation
  • Cumulative Time  : Operating Time

Pump

  • Pump 1 (acid)    : Set value and Present value (㎖)
  • Pump 2 (base)   : Set value and Present value (㎖)
  • Pump 3 (feed)    : Set value and Present value (㎖)
  • Pump 4 (foam)  : Set value and Present value (㎖)
Graphic Screen
  • Motor Agitation status
  • pH, DO, Temp. Sensor operation status
  • Foam sensor operating status
  • Pump operating status

Main Menu

  • Calibration Menu
  • Setup Menu
  • Control Menu
  • Data Log Menu
  • System Menu
  • Run On/Off

Information of Operating Status

  • Product Information : Product Name, Program Version etc.
  • Date & Time Set         : Year, Month, Day, Hour, Minute, Second
BIOEXCEL M Main - 수평
Explanation of BIOEXCEL M Fermentor Vessels

Single Vessel

Vessel01
  • It is used for microorganism cultivation and high speed agitation
  • (It is not used for animal cell fermentation as rpm speed should be slow)
  • Single vessel is relatively cheaper than double vessel
  • Low risk of glass damage from shock..
  • Temperature: single vessel does not require outside temperature control equipment.

Doubel Vessel

Double vessel
  • Heat transfer is stable due to larger heat transfer surface area.
  • Useful for quick cooling and decreasing temperature.
  • Water Bath is required for temperature control.
  • No congested area due to round shape bottom.

Bowl Vessel

Bowl
  • Bottom part is stainless.
  • Low risk of damage from shock.
  • Production capacity : Maximum total volume 19L.

Characteristics of heat :

  • Heat transfer surface area is larger than single vessel
  • Temperature cooling is faster than single vessel
  • It is a suitable combined form between single vessel and double vessel, and requires temperature controlling equipment (chiller or water bath)
  • Bottom stainless part is double jacket which can control vessel inside temperature by heating or cooling, so cold finger is not required. For huge volume, bowl vessel is most appropriate choice

Small Volume Vessel

Small Vol. Vessel
  • Small volume vessel below 1L are primarily for animal or stem cell cultivation.
  • As surface area of head plate is very limited on vessel below 1L, pH, DO, Temp, Foam, ORP Sensors, and etc. cannot be equipped and used on the top of the vessel.
  • In order to overcome such problem, sampling, condenser, feeding ports, and sensor ports are inserted in the side of the vessel as the form of several glass tubes with certain gradient.
  • The number, as well as size and shape, of side glass tubes can be adjusted according to customer’s request.
  • Customized design of the vessel can be offered according to the micro-carrier the user selected for high density cell cultivation.
  • The vessel can be customized for both adherent cell and suspension cell culture. Also, spin filter or depth filter type is available.
  • For stem cell cultivation, customized design is also available for assembling form of vessel with different heights of flat type or insertion of membrane inside the vessel.
Main Image of BIOEXCEL M Lab Scale Fermentor Accessories

Agitation Motor

agitation_motor150

Impeller

Ruston Turbin Impeller 3-a

Agitation Shaft

Vessel Shaft and Housing 1-a

Foam Breaker

Foam Breaket 3 set-a

Peristaltic Pump

Peristaltic Pump

Regent Bottle

Reagent Bottle

Condensor

콘덴서 1

Cold Finger

U type Cold Finger 1-a

Air Flow Meter

Air Flow Meter

Air Filter

Air Filter

Sparger

Ring Sparger 2-a

Air Compressor

Air compressor 10L

DO Sensor

DO Electrode

pH Sensor

ORP Sensor

ORP Sensor

ORP Sensor

Foam Sensor

Antifoam sensor

Temp. Sensor

Temperature Sensor

Sensor Cable

pH Cable

Top Plate

베셀상판 수정

Feeding Port

Rubber Stopper for 4 Feeding 3-a

화염접시

Inoculum Dish 1-a

Vessel Ports

Sensor Port Nut-a

Rubber Stopper

Rubber Stopper for Temp sensor Pipe-a

Sampling

sampling Unit

4 Gas Mixer

Gas Mixer

Reference : Bioreactor Technology, Sales Reference
BIOEXCEL M Type 6 Single Vessel
BIOEXCEL M Type 3 Single Vessel
BIOEXCEL M Type 3 Double Vessel
BIOEXCEL M Type 3 Continuous Culture
BIOEXCEL M Type 4 Single Vessel Old
BIOEXCEL M Type 3
Bioreactor Technology

Oxygen Transfer

Oxygen Transfer
All our hosts are aerobic, O2 is preferred terminal electron acceptor. Many promoters are down-regulated at low DO2 levels. O2 transfer is the major constraint in most of our fermentations!
Factors affecting O2 transfer in fermentors:
  • Viscosity (fungal – morphology – time-varying)
  • Agitation rate
  • P/V ratio
  • Tank/impeller geometry
  • Airflow rate (composition)
  • Media composition – surfactants (antifoams)
  • Fed-batch allows control of growth and O2 demand

Lab Scale Fermentor BIOEXCEL M Vessels

Single Vessel

Order No. Vessel Vol. (L)
1-VS-50m
50m
1-VS-100m
100m
1-VS-200m
200m
1-VS-300m
300m
1-VS-500m
500m
1-VS-1.5L
1.5L
1-VS-3L
3L
1-VS-5L
5L
1-VS-7L
7L
1-VS-10L
10L
1-VS-14L
14L
1-VS-19L
19L
9P-1
Top Plate (pH, DO, anti-foam, Thermo well, Inoculation, Sampling 4point feeding ports) Ports Cap, Blade, Foam Breaker, Pyrex Glass condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel silicon Tube, Vessel Packing, cold Finger Heating Plate (Heater, Water in/out ports)
  • Type : Single Wall and Flat Bottom
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Double Vessel

Order No. Vessel Vol. (L)
1-VD-1.5L
1.5L
1-VD-3L
3L
1-VD-5L
5L
1-VD-7L
7L
Double vessel
Pyrex Glass double Vessel Assembly with Vessel supporter Top Plate (pH,DO,Anti-foam, Thermo well, Inoculation, Sampling 4poin feeding points). Ports, Blade, Foam Breaker Pyrex Glass Condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Seal, Pyrex Glass Vessel Silicon Tube, Vessel packing, Cold Finger Heating Plate(Heater, water in/out ports) * Double vessel doesn’t need Heating Plate but Water bath for exclusive use
  • Type               : Double Wall and Dished Bottom type
  • Vessel material  : Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Bowl Vessel

Order No. Vessel Vol. (L)
1-VB-3L
3L
1-VB-5L
5L
1-VB-7L
7L
1-VB-10L
10L
1-VB-14L
14L
1-VB-19L
19L
1-VS-14L
14L
1-VS-19L
19L
Bowl
Pyrex glass single Vessel with BOWL type low plate Top Plate (pH, DO, Anti-foam, Thermo well, Inoculation, Sampling 4 point feeding ports SUS ports Cap, Blade, Foam Breaker Pyrex Glass Condenser, Ring type sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel Silicon Tube, Quick coupling, Vessel Packing Heating Plate (Heater, Water in/out ports)
  • Type               : Single Wall and Bowl Bottom
  • Vessel material  : Single well – Borosilicate glass, Bowl – SUS316L
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Small Volume Vessel

Order No. Vessel Vol. (L)
1-VSV-50m
50m
1-VSV-100m
100m
1-VSV-200m
200m
1-VSV-300m
300m
1-VSV-500m
500m
1-VSV-1000m
1000m
Small Vol. Vessel

Single Round Vessel

Order No. Vessel Vol. (L)
1-VSR-500m
500m
1-VSR-1L
1L
1-VSR-2L
2L
1-VSR-3L
3L
1-VSR-5L
5L
1-VSR-7L
7L
1-VSR-10L
10L
1-VSR-14L
14L
1-VSR-19L
19L
Animal cell culture Vessel 3L
Mass cultivation of animal cells has intensively been investigated even thought cell culture techniques have been developed for long times. There have been several difficulties in maintaining large quantities of live cells, especially more than million of cells per m/ in over five liter vessel. Fragility of animal cell, sophisticated culture conditions less economical feasibility of being scaled-up were major bottle necks in idustrializing cell culture technologies to produce a large amount of pharmaceuticals, tPA, interferon, EPO and many other MAb.
  • Type : Single round Vessel
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Lab Scale Fermentor BIOEXCEL M : Main Accessories


Lab Scale Fermentor BIOEXCEL M : Detail function of Operation Program on Bioreactor Controller

DO Control Mode
  • Cascade Select
  • RPM Control Setup, Gas Control Setup, Air Flow Control Setup,
  • RPM, Gas, Air Flow,
DO Control Mode : RPM Setup
  • Action Delay Time, Maximum RPM, Increase Step, Increase Time
  • Stable RPM Return
  • RPM After Gas Insert
DO Control Mode : Gas Setup
  • O2 Gas On/Off Time setup, Use/Not Use
  • N2 Gas On/Off Time setup, Use/Not Use
  • CO2 Gas On/Off Time setup, Use/Not Use
DO Control Mode
DO Control-RPM Setup
DO Control-Gas Setup
pH stat fed Batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, pH Control Use/Not Use
DO stat fed batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, DO Control Use/Not Use
Fed Batch Setup(pH Stats)
Fed Batch Setup(DO Stats)
pH Calibration 
  • 4 pH, 7 pH, 10 pH, Measuring pH, Temperature and mV
  • Temperature Compensation, Auto Calibration, Auto Calibration Processing Status
  • pH Calibration Status : Use Buffer, Slope, Run Calibration Status, Last Calibration Date
  • pH Recalibration : Process Value, Recalibration Set Value
  • pH Calibration End : Notice, 1 Point Calibration/pH is Not Calibration
DO Calibration
  • Zero, Sat
  • Temperature Compensation, Auto Calibration
  • DO Calibration Status : Last Calibration Date, Zero mV, Calibration Temp., Span mV, Max. DO
Temperature Calibration
  • Present Value, Set Temperature Input
  • Pump Calibration Acid Pump Calibration : Active Time Input, Input Volume(㎖), Pump On Start
  • Base Pump Calibration : Active Time Input, Input Volume(㎖), Pump On Start
  • Foam Pump Calibration : Active Time Input, Input Volume(㎖), Pump On Start
pH Calibration2
DO Calibration2
Temperature Calibration2
Setup Menu
  • pH Setup, DO Setup, Temperature Setup, RPM Setup, Foam Setup, Feed Setup, Alarm Setup
  • pH Setup : pH Set Value
  • Pump Set : Acid On/Off Time, Base On/Off Time
  • DO Setup : DO Set Value Input
  • Temperature Setup : Temperature Set Value Input
  • RPM Setup : Agitation Set Value Input
  • Foam Setup : Foam Set Value Input
  • Pump Set : Pump On/Off Time
  • Feed Setup : Pump Set On/Off Time
Alarm Setup
  • pH Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Acid Alarm Use : Set value(㎖)
  • Base Alarm Use : Set value(㎖)
  • Foam Alarm Use : Set value(㎖)
  • Feed Alarm Use : Set value(㎖)
  • DO Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Temperature Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • RPM Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
Setup Menu
Alarm Setup
Data Log
  • Data Log Setup, Report View, Real Time Graph Trend
Data Log Setup
  • Data Log Destination : PC, Flash Memory, Recorder, Printer
  • PC : Data Log Time Interval, User Name or Description
  • Data Log Item : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time
  • Flash Memory : Data Log Time Interval, User Name or Description
  • Data Log Item : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time
Data Acquisition
  • Selective Data gathering
  • Flash Memory : Max. 1,000,000 data
  • Real time Graphic Trend
  • Real time Data checking
  • Excel Data to PC
DataLogMenu
DataLogSetup
BIOEXCEL M Graph Screen 1
System Configuration
  • pH Control Configuration, Temperature Control & Sensor Configuration, Anti Foam Configuration, DO Sensor & Gas Configuration, Motor Select Configuration, Pump(Kind) Configuration, Communication Configuration, Touch Calibration, Net Work Configuration, Control Board Configuration, Protocol Configuration, Remote Control Configuration, H/W Module, Use/Not Use
System Test : Communication Test
  • Status Display, Receipt Information Display, 1 Packet Submit, 1 Packet Receipt, ACK Code, NACK Code, Automatic Submit and Receipt, ID
Temperature Test
  • PID Control Setting, Temperature control constant adjustment, Graphic Checking Display
H/W Port Test
  • Pump : Acid, Base, Feed, Foam, Gas1, Gas2, On/Off Checking Temperature : Heater, Cooler, On/Off Checking Motor : Set Value, RPM Read Checking
통신(Protocol)테스트
온도테스트
HW Test
System
  • Diagnostic, System Configuration, Advanced User Configuration
Diagnostic
  • Communication Test(Protocol Test), Temperature Test, Pump Test(Variable/Constant), H/W Port Test, Motor Test(Inverter/PWM), Flash Test, Inverter selection
Diagnostics Menu
System Configuration
Advanced User Configuration
Advanced User Configuration
  • pH Configuration, DO Configuration, Temperature Configuration, Other
Advanced User Configuration other
Advanced User pH Configuration
Advanced User DO Configuration
Advanced User Temp Configuration

Lab Scale Fermentor BIOEXCEL M : Detail Screen of Operation Program on Bioreactor Controller


Calibration Menu

Control Menu

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Setup Menu

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SYSTEM MENU : Advanced User
System Config
System Diagonostic
Progrma Map of BIOEXCEL M Bioreactor and Fermentor Controller
controller 화면 한글설명
Main Menu 설명
Setup Mene 설명
Calibration Menu 설명
System Menu 설명
Advanced User Configuration 설명
Data Log Menu

DataLogMenu
Data Log Setup

DataLogSetup
Graph Screen

BIOEXCEL M Graph Screen 2

Lab Scale Fermentor BIOEXCEL M : Fermentation Culture Method

Batch Culture

Batch Culture

A closed system initiated by the inoculation of microorganisms under certain environmental conditions such as specific nutrients, temperature, pressure, aeration and so on. Only few generations of microorganisms can grow before given nutrients are vanished.

  1. Closed system : only open to air
  2. Surface culture, submerged culture
  3. Parameter monitor & control
  4. Biomass growing up
Fed-Batch Culture

Fed-Batch

One or more nutrients (substrates) are fed to the bioreactor during cultivation and in which the product or cultured broth remains in the bioreactor until the end of the run.

  1. Open system
  2. High cell density culture
  3. Extended cultivation time
  4. Increase cell efficiency
Continuous Culture

Continuous culture

Cell culture method which keeps culture volume constant. Fresh medium flows into bioreactor continuously and part of the medium in the reactor is withdrawn from the bioreactor at the same flow rate of the inlet flow

  1. Chemostat : constant chemical environment characteristic of the steady state
  2. Control the growth rate with feeding rate
  3. Strain selection: faster growth
  4. Contamination : strict maintenance
  5. Cell physiology research
Fresh growth medium is added continuously during fermentation and spent medium is removed. (or cells itself are removed)