BIOEXCEL A

Autoclavable Lab Scale Bioreactor

Animal Cell Culture BIOREACTOR


BIOEXCEL A

Animal Cell Culture BIOREACTOR

Animal Cell Culture?

Cell culture has become one of the major tools used in the life science today. Animal cell culture was first successfully undertaken by Ross Harrison in 1907, since then, steadily enlarged its research territory through development in science. First, there was the development of antibiotics that made it easier to avoid many of contamination problems that plagued earlier cell culture attempts. Second was the development of the techniques, such as the use of trypsin to remove cells from culture vessels, necessary to obtain continuously growing cell lines (such as HeLa cells). Third, using these cell lines, scientists were able to develop standardized, chemically defined culture media that made it far easier to grow cells. These three areas combined to allow many more scientists to use cell, tissue and organ culture in their research.

BIOEXCEL A Bioreactor!

Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Despite these difficulties, various types of animal cells, including both undifferentiated and differentiated ones, can be cultured successfully. Cell culture is used for model systems, toxicity testing, cancer research, virology, cell-based manufacturing, genetic counseling, gene therapy and drug screening and development. Its business area is getting widened into pharmaceutical and bio-therapeutical industries.
BIOEXCEL A system is a lab scale bioreactor designed for multipurpose animal cell culture. It is an outstanding animal cell culture bioreactor system based on interactive control system. And the bioreactor system can offer various types of vessels such as Single round bottom vessel, Spin filter and Depth filter vessel, Double welled vessel, Small volume vessel, ranged from 50ml to 19L volume. As BIOTRON can re-design these various vessels according to customers’ demand, customers can obtain the best animal cell culture lab environment. Moreover, we can offer additional accessory items and external device, including micro carrier and disposable bag, thus constructing inclusive animal cell culture system.

Characteristic of Animal Cell

Unlike bacteria with cell wall, animal cell has only weak plasma membrane. Thus it is very sensitive to shear forces by bioreactor agitation or continuous stimulation of bubbles by supplied air/gas. Size of animal cells are approximately 10-100µm, relatively bigger than bacteria and dT(double time) is very slow, 18-28 hours. (Bacteria has 0.5-5 hours dT). Hence, animal cell requires very strict cell growth environment.

 The most important property of cell defining cell culture method is whether it is adherent cell or suspension cell. Adherent cell needs to be attached on the surface in order to grow, while suspension cell can do suspension cell culture. Normally, a cell maintaining adherence is much more stable condition, and when a cell changes to be suspension, it obtains cancer cell property.  This is because most cells except blood fluid or lymph are adherent cells. Most cells are adherent cell. However, one of most frequently used cell in bioreactor, Chinese Hamster Ovary (CHO) Cell, is processed through medium modification and adaption and then can be suspension cell cultured. Hybridoma can naturally suspension cell cultured.

For Scale-up, suspension cells are usually used in order to maintain maximum cell density volume per unit. CHO cell, normally used for medicine production, is adherent cell at first, but can be adapted to be suspension cell. They are easy to be scaled up and maintaining high density cell concentration, but may not supply nutrient and oxygen to cell inside cluster formed during cultivation. Hence, for animal cell culture scale up, it should not damage cell and must supply oxygen well. Oxygen has very low solubility in the medium. So supplying oxygen effectively to the animal cell inside culture medium is pivotal.(oxygenation)

Depth filter Vessel

Characteristic of animal cell is its sensitivity to shear stress due to large size and no cell wall, unlike microorganism. Inside bioreactor, hydrodynamics shear stress by agitation or aeration does damage cell. Mechanisms can be cell crash by each other or stirrer and baffle, pressure difference between impeller front and back, and eddy. Among them, main causes are formation of eddy and air bubble burst. When air bubble bursts, cell around it experiences huge shear stress. Therefore, in order to prevent cell damage, low aeration rate and gentle agitation are crucial for large volume bioreactor. Hollow fiber reactor has numerous fibers with bundle shape. The space and stoma between fiber and fiber bind cells in large space for growth. Medium penetrates through hollow separated by membranes continuously, thus supplying to the cell. Also, the resultant produced during cell growth, lactic acid, or ammonia passes through membrane and comes out to the medium with proliferation. At this time, cell concentration reaches 108 cells/ml and this can be operated more than one month. It can be used not only adherent cell, but also suspension cell such as hybridoma cell.

In case of suspension cell, suspension cell culture is commonly used for efficient production of monoclonal antibody with hybridoma cell, and adherent cell uses immobilization cultivation using micro-carrier or equivalent carrier for mass culture. For micro-carrier culture, dozens of micro-carrier are currently researched and used commercially. The shape of bioreactor is a stirred tank, and maximum cell density is 2-3 X 106 cells/ml by using 3-5% of micro-carrier. Especially, macro-porous micro-carrier can increase maximum cell density to 107 cells/ml. In order to increase productivity, medium recycling type perfusion continuous culture is commonly used. In this case, the technological development which can trap cell in the bioreactor, continuously supply only medium, and collect them and high medium supply speed are required.

BIOTRON applies spin filter for recycling type perfusion continuous culture. In most case, lab can also achieve obtain above 107cells/ml and 300-500µg/ml of antibody recovery. However, for pilot scale bioreactor, stability of bio-process, contamination possibility, and cell retention technology are really imperative.

Spin filter Vessel

BIOEXCEL A [Animal Cell Bioreactor] Specification Table

Summary of features and strength
  • BIOEXCEL M is a sensational concept of fermentation based on interactive control system.
  • BIOTRON’s BIOEXCEL M Fermentation system is a lab scale fermentor designed for multipurpose microorganism fermentation and cell culture.
  • This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience.
  • BIOEXCEL M bioreactor’s versatile vessels are suitable for optimum cultivation of bacteria, yeast or fungi, microorganism with optional accessories adapted for animal cell, plant cell, and microalgae. Batch, fed-batch, and continuous culture are available.
  • BIOEXCEL controller offers various bioreactor functions.
  • Color touch screen and easy to use GUI, self-process stage control on user basis and data logging in PC functions are adopted.
  • Core competence of BIOEXCEL M is providing perfect control reflecting the newest IT trend and changeover ideas for users to develop their own methods.
  • Easy to do Batch, Fed-Batch, Continuous culture fermentation, and it enables to cultivate micro-organism by accurate adjustment of temperature, agitation speed, pH, DO, and foams (which generate during the fermentation) via microprocessor and PC.
  • BIOEXCEL M Controller PID controls pH, Temperature, Agitation speed, and DO by Cascade method.
  • Cascade method is adopted for agitation speed or mixing gas. Fed-batch culture are available based on pH, DO, Glucose value. This fed-batch culture method maintains vessel inside condition based on constant level of pH, Do, and Glucose. Continuous culture can be also used as continuous feeding is available in the system.
  • Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps (acid, base, antifoam and feed)
  • Controller includes 4 peristaltic pumps for Acid, Base, Antifoam, and feeding for fermentor.
  • Various parameters such as pH, DO, Temperature, Agitation speed, Foam, and ORP can be controlled and monitored by sensors and controller.
  • BIOEXCEL M bioreactor’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement: 180mm (W), 15kg, and slim design.
  • BIOEXCEL M control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication.
  • Generally, motor for agitation is installed in top plate of vessel, and simple and durable agitation motor is used. In some case, Bottom magnetic Drive system can be applied.
  • Various size options are available for Single vessel, Double wall vessel, Bowl vessel, Single round bottom vessel, and Small volume vessel.
  • For cell culture such as animal or plant cell, servo motor can be used for agitation in very slow speed with accurate control of speed.
  • Special designed DC motor by BIOTRON can be accurately controlled within 50 ~ 850rpm.
  • Digital PID control algorithm can control the temperature very fast, and stable temperature is maintained.
  • pH value is calibrated in consideration of temperature, and can be controlled by feeding acid and base during fermentation
  • DO value is adjusted to measure accurate value according to temperature and salinity.
  • Ring shape sparger is made with stainless steel 316L, and many holes make consistent distribution of form
  • Additional exterior 4 channel gas mixer can be used.
Features
  • BIOEXCEL A Bioreactor is an outstanding animal cell culture bioreactor system based on interactive control system.
  • BIOEXCEL A system is a lab scale bioreactor designed for multipurpose animal cell culture.
  • There are various types of vessels for BIOEXCEL A Bioreactor, such as single round bottom vessel, spin filter and depth filter vessel, double welled vessel, small volume vessel, ranged from 50ml to 19L.
  • BIOTRON BIOEXCEL A Bioreactor system is a lab scale bioreactor designed for multipurpose cell culture.
  • The size of vessel can be decided upon the working volumes of real media and we provide interchangeable vessels that are convenient to scale up vessel among the various size of vessels.
  • Qualified for High density animal cell culture : Batch, DO-stat and pH-stat fed-batch, continuous culture, etc. with microprocessors controlling Temp, pH, DO, agitation and peristaltic pumping for various fermentation procedures on the control panel.
  • BIOTRON Control system shows the measured values of Temperature, pH, DO, Foam, ORP, Motor speed and the status of peristaltic pumps at the same time.
  • Monitor and manage data from anywhere! Save and manage data on a PC by simply connecting the controller to a PC.
  • The graphic user interface program tracks the status of the fermentation system in real time.
  • You can use 4 peristaltic pumps for feeding as well as external pumps.
  • All measured data and control parameters are transmitted to a PC by RS-232C and wireless communication protocol.
  • The BIOEXCEL A bioreactor’s 7-inch wide control panel screen, Built-in 7” wide high resolution color touch screen; easy-to-learn controller with intelligent GUI.
  • Sensor port and communication port are located in the front for the user’s convenience and allows side by side placement: 180mm (W), 15kg, and slim design. Side-by-side location of vessel and controller: 4 peristaltic pump and pH, DO, foam, temperature sensor port.
  • Easy to calibrate temperature, pH, DO, foam sensors when using graphic calibration mode.
  • The motor speed can be controlled by the DO value in DO mode to control the dissolved oxygen level. Agitation motor speed controlled by Dissolved oxygen rate in the DO mode automatically.
  • User can control fed batch by DO mode or accelerate motor speed Fed batch function and motor speed controls can be controlled by DO control mode.
  • User can choose feeding time so as to have stability and controllability.
  • The pH and DO value precisely with the temperature, and the value of dead-band for the peristaltic pump control can be adjusted. pH, DO offset: A operating instant of pH can be setting up by user, which resolve any control problems are occurred. User can choose feeding time so as to have stability and controllability. DO value from Dead band lead to certain point of feeding.
  • Intelligent self-diagnostic system to maintain fermentation process from start to end: Diagnostic, Setup, Dead Band, Offset, Warning, Feeding condition.
  • Pure glass touchscreens for superior image clarity durable, scratch resistant glass surface Finger, gloved hand, soft stylus activation Fast sensitive touch response Flat, spherical, cylindrical touchscreen,
  • By making additional use of ORP (Oxidation Reduction Potential) sensor with pH and DO sensor, you can monitor oxidation-reduction reaction in the vessel. This can be mainly used when checking the reduction of anaerobic culture.
  • A gas mixer can be used. If you connect BIOEXCEL A controller to an external 4 gas mixer, you can monitor and control the amount of the 4 types of gas (air, N2, O2, CO2).
  • Adjusting Feeding rate is most important factor for continuous culture. You can easily control feeding and discharge rate on BIOEXCEL A controller.
  • Multiple usage of peristaltic pump is possible. The BIOEXCEL A software allows the user to appoint the purpose without any need to change the array of 4 embedded peristaltic pump.
  • Trend Data Viewer: BIOEXCEL A controller shows parameter data such as pH, DO, Temperature, Agitation, Pump status during fermentation process in real time. For PC, this data is displayed as graph and past graph is accumulated and showed on the monitor.
  • Graph check and PID Value adjustment for temperature Test. By adjusting PID according to install environment, accurate temperature control is available. Temperature control test function shows actual temperature control situation on the graph.

BIOEXCEL M (RED) with single Vessel and 4 Gas Mixer 1

BIOEXCEL M (White) with single Vessel and 4 Gas Mixer 1
Specifications
Agitation Drive Top drive motor (mechanical drive)
Range 50~1,200 rpm
Sensor Magnetic hall sensor
Impellers 2-Rushton turbine impeller, 1-foam breaker
Indication Monolitic LCD display
Temperature Range From 3℃ above tde cooling water temperature to 80
(accuracy ±0.1℃)
Control Digital temperature controller
Sensor RTD(Pt-100)sensor
Aeration 2 Gas Mode Air, O2
4 Gas Mode Air, O2, CO2, N2,(in cell culture, in plant cell culture)
Air Flow, Electronic TMM or TMC
Sparger Ring sparger
Inlet Filter O.2㎛ air filter cartridge
Exhaust System Filter O.2㎛ air filter cartridge
Condenser SUS316
pH Setpoint / Display Range 2.0 ~ 12.0
Control Modes PID maintain witd 0.01 pH of setpoint
Electrode Mettler Toledo or Hamilton electrode
DO Setpoint Range 0 ~ 200% ±0.1
Display Range 0 ~ 200% ±0.1
Control Modes PID
Electrode Mettler Toledo or Hamilton electrode
Control Cabinet Dmension 250 D x 350 W x 300 H(mm)
Power 220V, 50/60Hz
Peristaltic Pumps Control 4 pumps each assignable to a wide variety of function for control of acid, base, foam and feed• 2 variable speed pumps for gas, PWM control Selectable to pumps function
Recording Output pH, DO, ORP, Foam, Temperature,Agitation, RS-232C
Heating Plate Electric Heater 400 watt
Dimension 250 D x 300 W x 100 H(mm)
Control PID
Specifications of BIOEXCEL A Bioreactor Controller

Main Controller

  • CPU                              : 32Bit ARM9 CPU, 266MHZ
  • Main Memory            : 64MB SD-RAM
  • Secondary Memory   : 64MB Flash Memory, 128MB Flash Memory for Data Logging
  • Watch Dog Timer      : Recovery when the system is down suddenly.
  • Real Time OS             : Developed Real Time Operating System
  • Real Time Clock

Measurement System

  • pH/DO/Temp/Pressure/Balance/Foam measurement analog board only communicate with main controller
  • Separated board Box
  • RS422A communication method
  • All sensor with separated power

pH Measure and Control System

  • Measurement and display range : 0 ~ 14.00
  • Control        : PID
  • Control output : Peristaltic pump signal and Solenoid signal for gas mixer
  • maintained within 0.01 pH of set point Selectable dead band of 0.01pH also available via keypad
  • Three-point auto calibration
  • Buffer recognition at pH 4.00, 7.00 and 10.00 buffer values
  • Automatic temperature compensation for greater accuracy at varying temperatures.
  • Simultaneously display pH (or mV) and temperature readings.
  • mV measurement display allows checking electrode performance.

DO Measure and Control System.

  • Measurement and Display range : 0.0 – 20.0ppm or 0.0 – 100.0%
  • Temperature compensation : PT100 sensor
  • Measurement range : 0~150 ℃
  • Read dissolved oxygen in mg/L, ppm, or % saturation with simultaneous temperature display
  • Automatic temperature compensation
  • Simultaneously display mV and temperature readings.
  • mV measurement display allows to check electrode performance.

Temperature Measure and control System

  • Measuring range: 0 – 150℃, Sterilizable
  • Accuracy         : f.s. ± 0.3 %
  • Sensor           : PT100 ohm double ceramic sensor with ready mounted cable
  • Measuring principle : Linear resistance type

Foam / LEVEL

  • Conductivity method
  • Sensitivity adjusting kit
  • Motor Control Board
  • Motor       : DC Motor/AC Motor
  • Diagnostics : Inverter selection

Motor Control Board

  • Motor      : DC Motor/AC Motor
  • Diagnostics             : Inverter selection

Variable speed motor control

  • Variable Speed peristaltic pump can be equipped.
  • Diagnostics : Inverter selection

ORP Sensor

  • ORP sensor port.

4 GAS Mixer

  • Control 4 GAS Mixer easily on the controller.

BIOEXCEL M Red

BIOEXCEL M White
Summary of BIOEXCEL A Bioreactor Program

Measuring : Set value and Present value

  • Sensor          : pH, ORP, DO, Temperature,
  • Agitation      : RPM
  • Aeration       : Air(MFC), O2, CO2
  • Foam Sensitive

Time

  • Start Time              : Start of Fermentation
  • Cumulative Time  : Operating Time

Pump

  • Pump 1 (acid)    : Set value and Present value (㎖)
  • Pump 2 (base)   : Set value and Present value (㎖)
  • Pump 3 (feed)    : Set value and Present value (㎖)
  • Pump 4 (foam)  : Set value and Present value (㎖)

Gas Mixer

  • Gas 1 (O2)      : Set value and Present value
  • Gas 2 (CO2)   : Set value and Present value
  • Gas 3 (N2)      : Set value and Present value
  • Gas 4 (Air)      : Set value and Present value

Graphic Screen

  • Motor Agitation status
  • pH, DO, ORP, Temp. Sensor operation status
  • Foam sensor operating status
  • Pump operating status
  • Variable pump speed display : 2 Pumps
  • Aeration operation status

Main Menu

  • Calibration Menu
  • Setup Menu
  • Control Menu
  • Data Log Menu
  • System Menu
  • Run On/Off

Information of Operating Status

  • Product Information : Product Name, Program Version etc.
  • Date & Time Set         : Year, Month, Day, Hour, Minute, Second
BIOEXCEL A Main
Description of BIOEXCEL A [Animal cell Culture bioreactor] Vessels

Spin Filter Vessel

Single round vessel for spin filter

In order to increase productivity, medium recycling type perfusion continuous culture is commonly used. In this case, the technological development which can trap cell in the bioreactor, continuously supply only medium, and collect them and high medium supply speed are required. BIOTRON applies spin filter for recycling type perfusion continuous culture.

Depth Filter Vessel

Depth Fiter Vessel

Hollow fiber reactor has numerous fibers with bundle shape. The space and stoma between fiber and fiber bind cells in large space for growth. Medium penetrates through hollow separated by membranes continuously, thus supplying to the cell.

Single round Vessel

Biotron animal cell 3L
  • Single round bottom vessel is used for animal cell fermentation as low speed agitation
  • Single vessel is relatively cheaper than double vessel
  • Low risk of glass damage from shock..
  • Temperature: single vessel does not require outside temperature control equipment.

Doubel Vessel

Double vessel
  • Heat transfer is stable due to larger heat transfer surface area.
  • Useful for quick cooling and decreasing temperature.
  • Water Bath is required for temperature control.
  • No congested area due to round shape bottom.

Small Volume Vessel

Small Vol. Vessel
  • Small volume vessel below 1L are primarily for animal or stem cell cultivation.
  • As surface area of head plate is very limited on vessel below 1L, pH, DO, Temp, Foam, ORP Sensors, and etc. cannot be equipped and used on the top of the vessel.
  • In order to overcome such problem, sampling, condenser, feeding ports, and sensor ports are inserted in the side of the vessel as the form of several glass tubes with certain gradient.
  • The number, as well as size and shape, of side glass tubes can be adjusted according to customer’s request.
  • Customized design of the vessel can be offered according to the micro-carrier the user selected for high density cell cultivation.
  • For stem cell cultivation, customized design is also available for assembling form of vessel with different heights of flat type or insertion of membrane inside the vessel.
  • The vessel can be customized for both adherent cell and suspension cell culture. Also, spin filter or depth filter type is available.

Bowl Vessel

Bowl
  • Bottom part is stainless.
  • Low risk of damage from shock.
  • Production capacity : Maximum total volume 19L.

Characteristics of heat :

  • Heat transfer surface area is larger than single vessel
  • Temperature cooling is faster than single vessel
  • It is a suitable combined form between single vessel and double vessel, and requires temperature controlling equipment (chiller or water bath)
  • Bottom stainless part is double jacket which can control vessel inside temperature by heating or cooling, so cold finger is not required. For huge volume, bowl vessel is most appropriate choice
Main Image of BIOEXCEL A Lab Scale Bioreactor Accessories

Agitation Motor

agitation_motor150

Impeller

Ruston Turbin Impeller 3-a

Agitation Shaft

Vessel Shaft and Housing 1-a

Foam Breaker

Foam Breaket 3 set-a

Peristaltic Pump

Peristaltic Pump

Regent Bottle

Reagent Bottle

Condensor

콘덴서 1

Cold Finger

U type Cold Finger 1-a

Air Flow Meter

Air Flow Meter

Air Filter

Air Filter

Sparger

Ring Sparger 2-a

Air Compressor

Air compressor 10L

DO Sensor

DO Electrode

pH Sensor

ORP Sensor

ORP Sensor

ORP Sensor

Foam Sensor

Antifoam sensor

Temp. Sensor

Temperature Sensor

Sensor Cable

pH Cable

Top Plate

베셀상판 수정

Feeding Port

Rubber Stopper for 4 Feeding 3-a

화염접시

Inoculum Dish 1-a

Vessel Ports

Sensor Port Nut-a

Rubber Stopper

Rubber Stopper for Temp sensor Pipe-a

Sampling

sampling Unit

4 Gas Mixer

Gas Mixer

Reference : Bioreactor Technology, Sales Reference
BIOEXCEL M Type 6 Single Vessel
BIOEXCEL M Type 3 Single Vessel
BIOEXCEL M Type 3 Double Vessel
BIOEXCEL M Type 3 Continuous Culture
BIOEXCEL M Type 4 Single Vessel Old
BIOEXCEL M Type 3
Bioreactor technology related with animal cell culture

Oxygen Transfer

Oxygen Transfer
All our hosts are aerobic, O2 is preferred terminal electron acceptor. Many promoters are down-regulated at low DO2 levels. O2 transfer is the major constraint in most of our fermentations!
Factors affecting O2 transfer in fermentors:
  • Viscosity (fungal – morphology – time-varying)
  • Agitation rate
  • P/V ratio
  • Tank/impeller geometry
  • Airflow rate (composition)
  • Media composition – surfactants (antifoams)
  • Fed-batch allows control of growth and O2 demand

Vessel for animal cell culture bioreactor

Depth Filter Vessel


Hollow fiber reactor has numerous fibers with bundle shape. The space and stoma between fiber and fiber bind cells in large space for growth. Medium penetrates through hollow separated by membranes continuously, thus supplying to the cell. Also, the resultant produced during cell growth, lactic acid, or ammonia passes through membrane and comes out to the medium with proliferation. At this time, cell concentration reaches 108 cells/ml and this can be operated more than one month. It can be used not only adherent cell, but also suspension cell such as hybridoma cell.

Round bottom Vessel


Small volume vessel below 1L are primarily for animal or stem cell cultivation. As surface area of head plate is very limited on vessel below 1L, pH, DO, Temp, Foam, ORP Sensors, and etc. cannot be equipped and used on the top of the vessel. In order to overcome such problem, sampling, condenser, feeding ports, and sensor ports are inserted in the side of the vessel as the form of several glass tubes with certain gradient.
The number, as well as size and shape, of side glass tubes can be adjusted according to customer’s request. Customized design of the vessel can be offered according to the micro-carrier the user selected for high density cell cultivation. The vessel can be customized for both adherent cell and suspension cell culture. Also, spin filter or depth filter type is available.

Spin Filter Vessel


In order to increase productivity, medium recycling type perfusion continuous culture is commonly used. In this case, the technological development which can trap cell in the bioreactor, continuously supply only medium, and collect them and high medium supply speed are required. BIOTRON applies spin filter for recycling type perfusion continuous culture. In most case, lab can also achieve obtain above 107cells/ml and 300-500µg/ml of antibody recovery. However, for pilot scale bioreactor, stability of bio-process, contamination possibility, and cell retention technology are really imperative.

Lab scale Animal cell culture Bioreactor : BIOEXCEL A Vessels

Single Vessel

Order No. Vessel Vol. (L)
1-VS-50m
50m
1-VS-100m
100m
1-VS-200m
200m
1-VS-300m
300m
1-VS-500m
500m
1-VS-1.5L
1.5L
1-VS-3L
3L
1-VS-5L
5L
1-VS-7L
7L
1-VS-10L
10L
1-VS-14L
14L
1-VS-19L
19L
9P-1
Top Plate (pH, DO, anti-foam, Thermo well, Inoculation, Sampling 4point feeding ports) Ports Cap, Blade, Foam Breaker, Pyrex Glass condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel silicon Tube, Vessel Packing, cold Finger Heating Plate (Heater, Water in/out ports)
  • Type : Single Wall and Flat Bottom
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Double Vessel

Order No. Vessel Vol. (L)
1-VD-1.5L
1.5L
1-VD-3L
3L
1-VD-5L
5L
1-VD-7L
7L
Double vessel
Pyrex Glass double Vessel Assembly with Vessel supporter Top Plate (pH,DO,Anti-foam, Thermo well, Inoculation, Sampling 4poin feeding points). Ports, Blade, Foam Breaker Pyrex Glass Condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Seal, Pyrex Glass Vessel Silicon Tube, Vessel packing, Cold Finger Heating Plate(Heater, water in/out ports) * Double vessel doesn’t need Heating Plate but Water bath for exclusive use
  • Type               : Double Wall and Dished Bottom type
  • Vessel material  : Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Bowl Vessel

Order No. Vessel Vol. (L)
1-VB-3L
3L
1-VB-5L
5L
1-VB-7L
7L
1-VB-10L
10L
1-VB-14L
14L
1-VB-19L
19L
1-VS-14L
14L
1-VS-19L
19L
Bowl
Pyrex glass single Vessel with BOWL type low plate Top Plate (pH, DO, Anti-foam, Thermo well, Inoculation, Sampling 4 point feeding ports SUS ports Cap, Blade, Foam Breaker Pyrex Glass Condenser, Ring type sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel Silicon Tube, Quick coupling, Vessel Packing Heating Plate (Heater, Water in/out ports)
  • Type               : Single Wall and Bowl Bottom
  • Vessel material  : Single well – Borosilicate glass, Bowl – SUS316L
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Small Volume Vessel

Order No. Vessel Vol. (L)
1-VSV-50m
50m
1-VSV-100m
100m
1-VSV-200m
200m
1-VSV-300m
300m
1-VSV-500m
500m
1-VSV-1000m
1000m
Small Vol. Vessel

Single Round Vessel

Order No. Vessel Vol. (L)
1-VSR-500m
500m
1-VSR-1L
1L
1-VSR-2L
2L
1-VSR-3L
3L
1-VSR-5L
5L
1-VSR-7L
7L
1-VSR-10L
10L
1-VSR-14L
14L
1-VSR-19L
19L
Animal cell culture Vessel 3L
Mass cultivation of animal cells has intensively been investigated even thought cell culture techniques have been developed for long times. There have been several difficulties in maintaining large quantities of live cells, especially more than million of cells per m/ in over five liter vessel. Fragility of animal cell, sophisticated culture conditions less economical feasibility of being scaled-up were major bottle necks in idustrializing cell culture technologies to produce a large amount of pharmaceuticals, tPA, interferon, EPO and many other MAb.
  • Type : Single round Vessel
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Lab Scale Animal cell culture Bioreactor BIOEXCEL A : Detail function of Operation Program on Bioreactor Controller

DO Control Mode
  • Cascade Select
  • RPM Control Setup, Gas Control Setup, Air Flow Control Setup,
  • RPM, Gas, Air Flow,
DO Control Mode : RPM Setup
  • Action Delay Time, Maximum RPM, Increase Step, Increase Time
  • Stable RPM Return
  • RPM After Gas Insert
DO Control Mode : Gas Setup
  • O2 Gas On/Off Time setup, Use/Not Use
  • N2 Gas On/Off Time setup, Use/Not Use
  • CO2 Gas On/Off Time setup, Use/Not Use
DO Control Mode
DO Control-RPM Setup
DO Control-Gas Setup
pH stat fed Batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, pH Control Use/Not Use
DO stat fed batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, DO Control Use/Not Use
Fed Batch Setup
Fed Batch Setup(pH Stats)060710
Fed Batch Setup(DO Stats)

Calibration Menu

  • –  pH Calibration, DO Calibration, Temperature Calibration,
  • –  Pump Calibration, Gas Calibration, Pressure Calibration

pH Calibration

  • 4 pH, 7 pH, 10 pH, Measuring pH, Temperature and mV
  • Temperature Compensation, Auto Calibration, Auto Calibration Processing Status
  • pH Calibration Status        : Use Buffer, Slope, Run Calibration Status, Last Calibration Date,
  • pH Recalibration               : Process Value, Recalibration Set Value
  • pH Calibration End            : Notice, 1 Point Calibration/pH is Not Calibration

DO Calibration

  • Zero, Sat
  • Temperature Compensation, Auto Calibration
  • DO Calibration Status        : Last Calibration Date, Zero mV, Calibration Temp., Span mV, Max. DO

Temperature Calibration

  • Present Value, Set Temperature Input

Pump Calibration

  • Acid Pump Calibration       : Active Time Input, Input Volume(㎖), Pump On Start
  • Base Pump Calibration      : Active Time Input, Input Volume(㎖), Pump On Start
  • Foam Pump Calibration     : Active Time Input, Input Volume(㎖), Pump On Start
  • Feed Pump Calibration      : Active Time Input, Input Volume(㎖), Pump On Start

Variable Speed Pump Calibration

  • Tube Width, RPM Set, Flow Rate
  • Time Control Use : On/Off Time, Test
pH Calibration2
DO Calibration2
Temperature Calibration2
ORP Calibration Screen
Pump Calibration Menu
Setup Menu
  • pH Setup, DO Setup, Temperature Setup, RPM Setup, Foam Setup, Feed Setup, Alarm Setup
  • pH Setup : pH Set Value
  • Pump Set : Acid On/Off Time, Base On/Off Time
  • DO Setup : DO Set Value Input
  • Temperature Setup : Temperature Set Value Input
  • RPM Setup : Agitation Set Value Input
  • Foam Setup : Foam Set Value Input
  • Pump Set : Pump On/Off Time
  • Feed Setup : Pump Set On/Off Time
Alarm Setup
  • pH Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Acid Alarm Use : Set value(㎖)
  • Base Alarm Use : Set value(㎖)
  • Foam Alarm Use : Set value(㎖)
  • Feed Alarm Use : Set value(㎖)
  • DO Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Temperature Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • RPM Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
DO Control-Gas Setup
pH Control-Gas Setup
pH Setup
Setup Menu
Feed Setup
Foam Setup
Alarm Setup

Data Log

  • Data Log Setup, Report View, Real Time Graph Trend

Data Log Setup

  • Data Log Destination : PC, Flash Memory, Recorder, Printer
  • PC                                   : Data Log Time Interval, User Name or Description
  • Data Log Item              : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time
  • Flash Memory              : Data Log Time Interval, User Name or Description
  • Data Log Item              : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Recorder

  • Data Log Time Interval, User Name or Description
  • Data Log Item                   : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Printer

  • Data Log Time Interval, User Name or Description
  • Data Log Item  : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Locking Warning   : Login ID/Pass Word

DataLogMenu
DataLogSetup
BIOEXCEL M Graph Screen 1

System

  • Diagnostic, System Configuration, Advanced User Configuration

Diagnostic

Communication Test(Protocol Test), Temperature Test, Pump Test(Variable/Constant), H/W Port Test, Motor Test(Inverter/PWM), Flash Test, Inverter selection

System Test

Communication Test
  • Status Display, Receipt Information Display, 1 Packet Submit, 1 Packet Receipt, ACK Code, NACK Code, Automatic Submit and Receipt, ID
Temperature Test
  • PID Control Setting, Temperature control constant adjustment, Graphic Checking Display
H/W Port Test
  • Pump                 : Acid, Base, Feed, Foam, Gas1, Gas2, On/Off Checking
  • Temperature    : Heater, Cooler, On/Off Checking
  • Motor                 : Set Value, RPM Read Checking
System Configuration
  • pH Control Configuration, Temperature Control & Sensor Configuration, Anti Foam Configuration, DO Sensor & Gas Configuration, Motor Select Configuration, Pump(Kind) Configuration, Communication Configuration, Touch Calibration, Net Work Configuration, Control Board Configuration, Protocol Configuration, Remote Control Configuration, H/W Module, Use/Not Use
Advanced User Configuration
  • pH Configuration, DO Configuration, Temperature Configuration, Other
pH Configuration
Temp Control&Sensor Configuration
Temp Control&Sensor Configuration(060913)
System
  • Diagnostic, System Configuration, Advanced User Configuration
Diagnostic
  • Communication Test(Protocol Test), Temperature Test, Pump Test(Variable/Constant), H/W Port Test, Motor Test(Inverter/PWM), Flash Test, Inverter selection
온도테스트
System Configuration
Advanced User Configuration
Advanced User Configuration
  • pH Configuration, DO Configuration, Temperature Configuration, Other
Advanced User DO Configuration
Advanced User Configuration
Advanced User Configuration other(060613)
Advanced User Configuration Agitation(061221)
User Feeding Control Select
User Feeding Pump Control
User Feeding Gas Control

BIOEXCEL A Controller Program


Calibration Menu

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Control Menu

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Setup Menu

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SYSTEM MENU : Advanced User
System Config
System Diagonostic
Progrma Map of BIOEXCEL M Bioreactor and Fermentor Controller
controller 화면 한글설명
Main Menu 설명
Setup Mene 설명
Calibration Menu 설명
System Menu 설명
Advanced User Configuration 설명
Data Log Menu

DataLogMenu
Data Log Setup

DataLogSetup
Graph Screen

BIOEXCEL M Graph Screen 2

Cell Culture Method

Batch Culture
Batch Culture
A closed system initiated by the inoculation of microorganisms under certain environmental conditions such as specific nutrients, temperature, pressure, aeration and so on. Only few generations of microorganisms can grow before given nutrients are vanished.
  1. Closed system : only open to air
  2. Surface culture, submerged culture
  3. Parameter monitor & control
  4. Biomass growing up
Fed-Batch Culture

Fed-Batch

One or more nutrients (substrates) are fed to the bioreactor during cultivation and in which the product or cultured broth remains in the bioreactor until the end of the run.

  1. Open system
  2. High cell density culture
  3. Extended cultivation time
  4. Increase cell efficiency
Continuous Culture

Continuous culture

Cell culture method which keeps culture volume constant. Fresh medium flows into bioreactor continuously and part of the medium in the reactor is withdrawn from the bioreactor at the same flow rate of the inlet flow

  1. Chemostat : constant chemical environment characteristic of the steady state
  2. Control the growth rate with feeding rate
  3. Strain selection: faster growth
  4. Contamination : strict maintenance
  5. Cell physiology research
Fresh growth medium is added continuously during fermentation and spent medium is removed. (or cells itself are removed)
Perfusion Culture
Perfusion culture diagram
Perfusion system is one of the culture method which continuously provides fresh nutrients through pump, and separate certain amount of medium by using spin filter. When cell density increases high, it causes problem with cell growth, so some amounts of cells are removed, which is called bleeding. Generally, bleeding is conducted by removing 0.1 volume based on total bioreactor culture volume.
The advantage of perfusion culture is it can increase cell density and target protein density. Moreover, growth rate can be regulated by bleeding, regardless of nutrients supply. Most cells are placed inside the bioreactor, so at early growth stage, most nutrients are used for growth of biomass, but later on they can be controlled to be used for production of target protein. Purification is a stage between continuous and batch culture, that medium cells must be continuously removed. However, as there is a device which separates cells, it is really complicated, compared with general stirred tank bioreactor using chemostat. Furthermore, as viability of cell is relatively low, there is not only cell debris, but also blocking cell separating device and malfunctions of sensors when exceeding limit.