BIOEXCEL P

Autoclavable Bioreactor

Lab Scale Plant Cell Culture Bioreactor


BIOEXCEL P

Lab scale plant cell culture Bioreactor

SmartGX

Plant cell culture bioreactor!

Bioreactors have several advantages for mass cultivation of plant cells. First of all, it gives better control for scale up of cell suspension cultures under defined parameters for the production of bioactive compounds. Second, constant regulation of conditions at various stages of bioreactor operation is possible. Furthermore, handling of culture such as inoculation or harvest is easy and saves time. Also, nutrient uptake is enhanced by submerged culture conditions which Stimulate multiplication rate and higher yield of bioactive compounds. At last, number of plantlets are easily produced and can be scaled-up.
As the bioreactor is a physical/thermal system for maintenance of cells at the best culture conditions for a fast growth, several models can be considered as bioreactors, starting from simple close vessels that can be externally agitated up to complex aseptic systems controlled and regulated by appropriate software.

Plant Cell Culture?

Plants produce several different secondary metabolites, called phytochemicals mostly of them used as pharmaceuticals. In recent years biopharmaceutical/nutraceutical industry renewed in­creased attention in production of health‑promoting secondary metabolites using plant cell and tissue cultures.
Secondary metabolites produced in plant cells are either released into medium or accumulated in the cells. Thus the spent medium or the biomass is harvested after suitable incubation period for the extraction of bioactive compounds. Sometimes different media for growth and production are used to obtain maximum secondary metabolite production.
Cell growth and secondary metabolite production represent the main factors for selecting the suitable process mode.
The production of in‑vitro secondary metabolites can be possible through plant cell cultures. This technology represents a good model to overcome many problems linked to the conventional agriculture such as variations in the crop quality due to environmental factors

In alternative to wild collection or plant cultivation, the production of useful and valuable sec­ondary metabolites in large bioreactors is an attractive proposal; it should contribute significantly to future attempts to preserve global biodiversity and alleviate associated ecological problems. The advantages of such processes include the controlled production according to demand and a reduced man work requirement. Plant cells have been grown in different shape bioreactors, however, there are a variety of problems to be solved before this technology can be adopted on a wide scale for the production of useful plant secondary metabolites.

Characteristic of Plant cell culture Bioreactor

Such special features of cultured plant cells necessitate to design and develop a impeller for bioreactor, which could provide proper mixing and sufficient oxygen supply throughout the bioreactor without forming dead zones and will not damage the cells during cultivation period. The hydrodynamic conditions in bioreactors are most strongly dependent on the impeller design.
Different process modes are used, such as batch culture, fed batch culture, rapid feed batch culture, two‑stage batch culture and continuous culture.

Major factors in plant cell culture Bioreactor

There are different factors affecting the culture growth and secondary metabolite production in bioreactors: the gaseous atmosphere, oxygen supply and CO2 exchange, pH, minerals, carbohydrates, growth regulators, the liquid medium rheology and cell density. Moreover agitation systems and sterilization conditions may negatively influence the whole process. In order to manage the biomass growth in bioreactors, above mentioned factors must be controlled.
Many types of plant cell culture bioreactors have been successfully used for cultivating transformed root cultures, depending on both different aeration system and nutrient supply. Several examples of medicinal and aromatic plant cultures were here summarized for the scale up cultivation in bioreactors.

High aeration rates appear to reduce the biomass growth

In general, high aeration rates appear to reduce the biomass growth. High aeration rates were found to inhibit cell growth in cell suspensions cultured in airlift bioreactors. It is reported that high aeration rates rather than excessive oxygen inhibit growth and this reduction could be due to depletion of CO2 or to the removal of various culture volatiles including CO2. The requirement for CO2 was not related to photosynthesis but to some other metabolic pathways involved in amino acid biosynthesis.

Photoautotrophic Cell

Plants evolve CO2 and consume O2 during respiration, while during photosynthesis CO2 is used and O2 is produced. If photoautotrophic conditions persisted in the plant cell culture, CO2 levels increased during the dark period, while they decreased during the light period. The effects of CO2 were reported for both agarized cultures and cell suspension cultures used for secondary metabolite production.

Oxygen Transfer Coefficient

The amount of O2 in bioreactor depends on the presence of O2 in the gas phase above and in the air bubbles inside the medium, as well as on the dissolved O2 in the medium. Air is released through a sparger located at the base of the bioreactor. The available oxygen for plant cells in liquid cultures, determined by oxygen transfer coefficient (kLa values), is the part that dissolves in water. The level of O2 in liquid cultures in bioreactors can be regulated by agitation or stirring and through aeration, gas flow and air bubble size. The use of a porous irrigation tube as a sparger generated fine bubbles, high kLa values, low mechanical stress and provided a high growth rate.

Secondary metabolites through plant cell cultivation system

There are many such secondary metabolites that can benefit the living of people as a form of pigment, medicine or perfume. However, extracting these materials directly from plants is restricted: the content is low, productivity of each part of plant is different, and the growth of plant is limited and effected by temperature, season and place. These limitations can be overcome by plant cell cultivation system. The useful, and producible secondary metabolites through plant cell cultivation system are antitumor antibiotics (vincristine, vinblastine, podophyllotoxins , maytansine, tripdio-lide, homoharringt onine, paclitaxel, ellipticin, thalicarpin, baccharin, and bouvardin), sterol as cholesterol inhibitor , heart diseases related cures ubiquinone and antibiotics, shikonin for antiulcer, and saponin in ginseng. Currently, over 300,000 species of plants are known to have secondary metabolites, and every year materials from about 1,500 plant species are extracted and separated. 300 species are evaluated to have useful physiologically active materials.


BIOEXCEL P [Plant Cell Bioreactor] Specification Table

Summary of features and strength

BIOEXCEL P Plant cell culture bioreactor is a system for maintenance of cells at the best culture conditions for a fast growth, several models can be considered as bioreactors, starting from simple close vessels that can be externally agitated up to complex aseptic systems controlled and regulated by appropriate software.

Bioreactors have advantages for mass cultivation of plant cells. It gives better control for scale up of cell suspension cultures under defined parameters for the production of bioactive compounds.

Plant cell culture bioreactor must control gaseous atmosphere, oxygen supply and CO2 exchange, pH, minerals, carbohydrates, growth regulators, the liquid medium rheology and cell density, for culture growth and secondary metabolite production. Our agitation systems and sterilization conditions are designed appropriately in order to control these factors.

Our impeller of Plant cell culture bioreactor is designed and developed to provide proper mixing and sufficient oxygen supply throughout the bioreactor without forming dead zones, without damaging the cells during cultivation period. The hydrodynamic conditions in bioreactors are most strongly dependent on the impeller design.

Plant cell bioreactor may use different culture methods such as batch culture, fed batch culture, rapid feed batch culture, two stage batch culture and continuous culture.

Plant cell culture bioreactor has agitation systems and pneumatically agitation system.

Mechanically stirred bioreactors have a helical ribbon impeller, magnetic stirrers, or vibrating perforated plates and a flat blade turbine impeller, which high agitation breaks incoming air into small bubbles.

Pneumatically Agitated Bioreactors have two types: bubble column and air lift. These are tall and thin in comparison with agitation bioreactors. In the bubble columns air is bubbled at the base of the column, thus medium is agitated. In air lift bioreactors, gas is sparged from the riser section to the top of the column and the medium flows downward in the down corner section.

BIOEXCEL P Bioreactor system is a lab scale plant cell culture bioreactor. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. Usually, plant cell culture bioreactor with volume from 1L to 19L is characterized as lab scale.

BIOEXCEL M control system is basically applied for BIOEXCEL P Bioreactor. However, if user wants 4-gas mixer, ORP sensor, variable motor spped controller or any other optional functions, BIOEXCEL A controller can be applied.

The BIOEXCEL M’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication. The BIOEXCEL M’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement and slim design.

BIOEXCEL M Controller PID controls pH, Temperature, Agitation speed, and DO by Cascade method. Cascade method is adopted for agitation speed or mixing gas. Fed-batch culture are available based on pH, DO. This fed-batch culture method maintains vessel inside condition based on constant level of pH, DO. Continuous culture can be also used as continuous feeding is available in the system.

BIOEXCEL M Controller supports pH, Do, temperature and foam sensor. It also includes 4 peristaltic pumps for Acid, Base, Antifoam, and feeding for fermentor.

Easy to do Batch, Fed-Batch, Continuous culture fermentation, and it enables to cultivate micro-organism by accurate adjustment of temperature, agitation speed, pH, DO, and foams (which generate during the fermentation) via microprocessor and PC. Also, these three culture modes can be used for animal cell, plant cell, and microalgae, but please note that all functions which can satisfy customer’s every single demand cannot be offered.

Sensors controlling of pH, DO, temperature and foam with 4 peristaltic pumps(acid, base, antifoam and feed), DO, pH, Temp, Foam, sensors are available.

Generally, motor for agitation is installed in top plate of vessel, and simple and durable agitation motor is used. In some case, Bottom magnetic Drive system can be applied. Various size options are available for Single vessel, Double wall vessel, Bowl vessel, Single round bottom vessel, and Small volume vessel. For cell culture such as animal or plant cell, servo motor can be used for agitation in very slow speed with accurate control of speed.

Digital PID control algorithm can control the temperature very fast, and stable temperature is maintained. pH value is calibrated in consideration of temperature, and can be controlled by feeding acid and base during fermentation. DO value is adjusted to measure accurate value according to temperature and salinity.

Features (BIOEXCEL M Controller)
  • BIOEXCEL P Plant cell culture bioreactor is a system for maintenance of cells at the best culture conditions for a fast growth, several models can be considered as bioreactors, starting from simple close vessels that can be externally agitated up to complex aseptic systems controlled and regulated by appropriate software.
  • Bioreactors have advantages for mass cultivation of plant cells. It gives better control for scale up of cell suspension cultures under defined parameters for the production of bioactive compounds.
  • Our impeller of Plant cell culture bioreactor is designed and developed to provide proper mixing and sufficient oxygen supply throughout the bioreactor without forming dead zones, without damaging the cells during cultivation period. The hydrodynamic conditions in bioreactors are most strongly dependent on the impeller design.
  • Plant cell bioreactor may use different culture methods such as batch culture, fed batch culture, and continuous culture.
  • Plant cell culture bioreactor has agitation systems and pneumatically agitation system.
  • BIOEXCEL P Bioreactor system is a lab scale plant cell culture bioreactor. This model is recommended for researcher, professor and supervisor for diversified experimental methods as well as providing intellectual communication with convenience. Usually, plant cell culture bioreactor with volume from 1L to 19L is characterized as lab scale.
  • The BIOEXCEL P’s control system is easy to handle, and all the monitored data and control parameters are transmitted to a PC via serial communication.
  • The BIOEXCEL P’s 7-inch wide control panel screen, sensor port and communication port are located in the front and allows side by side placement and slim design.
  • Sensor port and communication port are located in the front for the user’s convenience and allows side by side placement: 180mm (W), 15kg, and slim design. Side-by-side location of vessel and controller: 4 peristaltic pump and pH, DO, foam, temperature sensor port.
  • BIOEXCEL P Controller PID controls pH, Temperature, Agitation speed, and DO by Cascade method. Cascade method is adopted for agitation speed or mixing gas.
  • Digital PID control algorithm can control the temperature very fast, and stable temperature is maintained.
  • pH value is calibrated in consideration of temperature, and can be controlled by feeding acid and base during fermentation.
  • DO value is adjusted to measure accurate value according to temperature and salinity.
  • The pH and DO value precisely with the temperature, and the value of dead-band for the peristaltic pump control can be adjusted.
  • Fed-batch culture are available based on pH, DO. This fed-batch culture method maintains vessel inside condition based on constant level of pH, DO. Continuous culture can be also used as continuous feeding is available in the system.
  • The motor speed can be controlled by the DO value in DO mode to control the dissolved oxygen level. Agitation motor speed controlled by Dissolved oxygen rate in the DO mode automatically.
  • User can control fed batch by DO mode or accelerate motor speed Fed batch function and motor speed controls can be controlled by DO control mode.
  • User can choose feeding time so as to have stability and controllability.
  • BIOEXCEL P Controller supports pH, Do, temperature and foam sensor. It also includes 4 peristaltic pumps for Acid, Base, Antifoam, and feeding for plant cell culture bioreactor.
  • Easy to calibrate temperature, pH, DO, foam sensors when using graphic calibration mode.
  • Generally, motor for agitation is installed in top plate of vessel, and simple and durable agitation motor is used. In some case, Bottom magnetic Drive system can be applied.
  • Various size options are available for Single vessel, Double wall vessel, Bowl vessel, Single round bottom vessel, and Small volume vessel. For cell culture such as animal or plant cell, servo motor can be used for agitation in very slow speed with accurate control of speed.
  • pH, DO offset: A operating instant of pH can be setting up by user, which resolve any control problems are occurred. User can choose feeding time so as to have stability and controllability. DO value from Dead band lead to certain point of feeding.
  • Intelligent self-diagnostic system to maintain fermentation process from start to end: Diagnostic, Setup, Dead Band, Offset, Warning, Feeding condition.
  • Trend Data Viewer: BIOEXCEL P controller shows parameter data such as pH, DO, Temperature, Agitation, Pump status during fermentation process in real time. For PC, this data is displayed as graph and past graph is accumulated and showed on the monitor.
  • Graph check and PID Value adjustment for temperature Test. By adjusting PID according to install environment, accurate temperature control is available. Temperature control test function shows actual temperature control situation on the graph.

BIOEXCEL M (RED) with single Vessel and 4 Gas Mixer 1

BIOEXCEL M (White) with single Vessel and 4 Gas Mixer 1
Specifications (BIOEXCEL M Controller)
Agitation Drive Top drive motor (mechanical drive)
Range 50~1,200 rpm
Sensor Magnetic hall sensor
Impellers 2-Rushton turbine impeller, 1-foam breaker
Indication Monolitic LCD display
Temperature Range From 3℃ above tde cooling water temperature to 80 (accuracy ±0.1℃)
Control Digital temperature controller
Sensor RTD(Pt-100)sensor
Aeration 2 Gas Mode Air, O2
4 Gas Mode Air, O2, CO2, N2,(in cell culture, in plant cell culture)
Air Flow, Electronic TMM or TMC
Sparger Ring sparger
Inlet Filter O.2㎛ air filter cartridge
Exhaust System Filter O.2㎛ air filter cartridge
Condenser SUS316
pH Setpoint / Display Range 2.0 ~ 12.0
Control Modes PID maintain witd 0.01 pH of setpoint
Electrode Mettler Toledo or Hamilton electrode
DO Setpoint Range 0 ~ 200% ±0.1
Display Range 0 ~ 200% ±0.1
Control Modes PID
Electrode Mettler Toledo or Hamilton electrode
Control Cabinet Dmension 250 D x 350 W x 300 H(mm)
Power 220V, 50/60Hz
Peristaltic Pumps Control 4 pumps each assignable to a wide variety of function for control of acid, base, foam and feed
Recording Output pH, DO, Foam, Temperature,Agitation, RS-232C
Heating Plate Electric Heater 400 watt
Dimension 250 D x 300 W x 100 H(mm)
Control PID

* Please note that technical specifications are subjected to be changed.

Specifications of BIOEXCEL M Bioreactor Controller

Main Controller

  • CPU                              : 32Bit ARM9 CPU, 266MHZ
  • Main Memory            : 64MB SD-RAM
  • Secondary Memory   : 64MB Flash Memory, 128MB Flash Memory for Data Logging
  • Watch Dog Timer      : Recovery when the system is down suddenly.
  • Real Time OS             : Developed Real Time Operating System
  • Real Time Clock

Measurement System

  • pH/DO/Temp/Pressure/Balance/Foam measurement analog board only communicate with main controller
  • Separated board Box
  • RS422A communication method
  • All sensor with separated power

pH Measure and Control System

  • Measurement and display range : 0 ~ 14.00
  • Control        : PID
  • Control output : Peristaltic pump signal and Solenoid signal for gas mixer
  • maintained within 0.01 pH of set point Selectable dead band of 0.01pH also available via keypad
  • Three-point auto calibration
  • Buffer recognition at pH 4.00, 7.00 and 10.00 buffer values
  • Automatic temperature compensation for greater accuracy at varying temperatures.
  • Simultaneously display pH (or mV) and temperature readings.
  • mV measurement display allows checking electrode performance.

DO Measure and Control System.

  • Measurement and Display range : 0.0 – 20.0ppm or 0.0 – 100.0%
  • Temperature compensation : PT100 sensor
  • Measurement range : 0~150 ℃
  • Read dissolved oxygen in mg/L, ppm, or % saturation with simultaneous temperature display
  • Automatic temperature compensation
  • Simultaneously display mV and temperature readings.
  • mV measurement display allows to check electrode performance.

Temperature Measure and control System

  • Measuring range: 0 – 150℃, Sterilizable
  • Accuracy         : f.s. ± 0.3 %
  • Sensor           : PT100 ohm double ceramic sensor with ready mounted cable
  • Measuring principle : Linear resistance type

Foam / LEVEL

  • Conductivity method
  • Sensitivity adjusting kit
  • Motor Control Board
  • Motor       : DC Motor/AC Motor
  • Diagnostics : Inverter selection

Motor Control Board

  • Motor      : DC Motor/AC Motor
  • Diagnostics             : Inverter selection

Variable speed motor control

  • Variable Speed peristaltic pump can be equipped.
  • Diagnostics : Inverter selection

ORP Sensor

  • ORP sensor port.

4 GAS Mixer

  • Control 4 GAS Mixer easily on the controller.

BIOEXCEL M Controller

BIOEXCEL M Red

BIOEXCEL A Controller

BIOEXCEL M White

BIOEXCEL M control system is basically applied for BIOEXCEL P Plant cell culture bioreactor. However, if user wants 4-gas mixer, ORP sensor, variable motor spped controller or any other optional functions, BIOEXCEL A controller can be applied.

BIOEXCEL M Controller Program
BIOEXCEL M Main

Measuring : Set value and Present value

  • Sensor          : pH, DO, Temperature
  • Agitation      : RPM
  • Foam sensitive
Time
  • Start Time              : Start of Fermentation
  • Cumulative Time  : Operating Time

Pump

  • Pump 1 (acid)    : Set value and Present value (㎖)
  • Pump 2 (base)   : Set value and Present value (㎖)
  • Pump 3 (feed)    : Set value and Present value (㎖)
  • Pump 4 (foam)  : Set value and Present value (㎖)
Graphic Screen
  • Motor Agitation status
  • pH, DO, Temp. Sensor operation status
  • Foam sensor operating status
  • Pump operating status

Main Menu

  • Calibration Menu
  • Setup Menu
  • Control Menu
  • Data Log Menu
  • System Menu
  • Run On/Off

Information of Operating Status

  • Product Information : Product Name, Program Version etc.
  • Date & Time Set         : Year, Month, Day, Hour, Minute, Second
BIOEXCEL A Controller Program
BIOEXCEL A Main

Measuring : Set value and Present value

  • Sensor          : pH, ORP, DO, Temperature,
  • Agitation      : RPM
  • Aeration       : Air(MFC), O2, CO2
  • Foam Sensitive

Time

  • Start Time              : Start of Fermentation
  • Cumulative Time  : Operating Time

Pump

  • Pump 1 (acid)    : Set value and Present value (㎖)
  • Pump 2 (base)   : Set value and Present value (㎖)
  • Pump 3 (feed)    : Set value and Present value (㎖)
  • Pump 4 (foam)  : Set value and Present value (㎖)

Gas Mixer

  • Gas 1 (O2)      : Set value and Present value
  • Gas 2 (CO2)   : Set value and Present value
  • Gas 3 (N2)      : Set value and Present value
  • Gas 4 (Air)      : Set value and Present value

Graphic Screen

  • Motor Agitation status
  • pH, DO, ORP, Temp. Sensor operation status
  • Foam sensor operating status
  • Pump operating status
  • Variable pump speed display : 2 Pumps
  • Aeration operation status

Main Menu

  • Calibration Menu
  • Setup Menu
  • Control Menu
  • Data Log Menu
  • System Menu
  • Run On/Off

Information of Operating Status

  • Product Information : Product Name, Program Version etc.
  • Date & Time Set         : Year, Month, Day, Hour, Minute, Second
Description of BIOEXCEL P Plant cell culture Bioreactor Vessels

Single round Vessel

Biotron animal cell 3L
  • Single round vessel is that vessel bottom is made of round shape, then  there is no stagnant area in vessel while agitator is running.
  • Heating Blanket 으로 vessel을 감싸서 온도를 조절한다. (30~40℃)
  • Single round bottom vessel is used for plant cell fermentation as low speed agitation
  • Single vessel is relatively cheaper than double vessel
  • Low risk of glass damage from shock..
  • Temperature: single vessel does not require outside temperature control equipment.

Doubel Vessel

Double vessel
  • Heat transfer is stable due to larger heat transfer surface area.
  • Useful for quick cooling and decreasing temperature.
  • Water Bath is required for temperature control.
  • No congested area due to round shape bottom.

 

Bowl Vessel

Bowl
  • Bottom part is stainless.
  • Low risk of damage from shock.
  • Production capacity : Maximum total volume 19L.

Characteristics of heat :

  • Heat transfer surface area is larger than single vessel
  • Temperature cooling is faster than single vessel
  • It is a suitable combined form between single vessel and double vessel, and requires temperature controlling equipment (chiller or water bath)
  • Bottom stainless part is double jacket which can control vessel inside temperature by heating or cooling, so cold finger is not required. For huge volume, bowl vessel is most appropriate choice

Single Vessel

9P-1
  • It is used for plant cell bioreactor as low speed agitation
  • Single vessel is relatively cheaper than double vessel
  • Low risk of glass damage from shock..
  • Temperature: single vessel does not require outside temperature control equipment.

 

Main Image of BIOEXCEL M Lab Scale Fermentor Accessories

Agitation Motor

agitation_motor150

Impeller

Ruston Turbin Impeller 3-a

Agitation Shaft

Vessel Shaft and Housing 1-a

Foam Breaker

Foam Breaket 3 set-a

Peristaltic Pump

Peristaltic Pump

Regent Bottle

Reagent Bottle

Condensor

콘덴서 1

Cold Finger

U type Cold Finger 1-a

Air Flow Meter

Air Flow Meter

Air Filter

Air Filter

Sparger

Ring Sparger 2-a

Air Compressor

Air compressor 10L

DO Sensor

DO Electrode

pH Sensor

ORP Sensor

ORP Sensor

ORP Sensor

Foam Sensor

Antifoam sensor

Temp. Sensor

Temperature Sensor

Sensor Cable

pH Cable

Top Plate

베셀상판 수정

Feeding Port

Rubber Stopper for 4 Feeding 3-a

화염접시

Inoculum Dish 1-a

Vessel Ports

Sensor Port Nut-a

Rubber Stopper

Rubber Stopper for Temp sensor Pipe-a

Sampling

sampling Unit

4 Gas Mixer

Gas Mixer

Reference : Bioreactor Technology, Sales Reference
BIOEXCEL M Type 6 Single Vessel
BIOEXCEL M Type 3 Single Vessel
BIOEXCEL M Type 3 Double Vessel
BIOEXCEL M Type 3 Continuous Culture
BIOEXCEL M Type 4 Single Vessel Old
BIOEXCEL M Type 3
Bioreactor technology related with animal cell culture

Oxygen Transfer

Oxygen Transfer
All our hosts are aerobic, O2 is preferred terminal electron acceptor. Many promoters are down-regulated at low DO2 levels. O2 transfer is the major constraint in most of our fermentations!
Factors affecting O2 transfer in fermentors:
  • Viscosity (fungal – morphology – time-varying)
  • Agitation rate
  • P/V ratio
  • Tank/impeller geometry
  • Airflow rate (composition)
  • Media composition – surfactants (antifoams)
  • Fed-batch allows control of growth and O2 demand

Vessel for Plant cell culture bioreactor

Single Vessel

Order No. Vessel Vol. (L)
1-VS-50m
50m
1-VS-100m
100m
1-VS-200m
200m
1-VS-300m
300m
1-VS-500m
500m
1-VS-1.5L
1.5L
1-VS-3L
3L
1-VS-5L
5L
1-VS-7L
7L
1-VS-10L
10L
1-VS-14L
14L
1-VS-19L
19L
9P-1
Top Plate (pH, DO, anti-foam, Thermo well, Inoculation, Sampling 4point feeding ports) Ports Cap, Blade, Foam Breaker, Pyrex Glass condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel silicon Tube, Vessel Packing, cold Finger Heating Plate (Heater, Water in/out ports)
  • Type : Single Wall and Flat Bottom
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Double Vessel

Order No. Vessel Vol. (L)
1-VD-1.5L
1.5L
1-VD-3L
3L
1-VD-5L
5L
1-VD-7L
7L
Double vessel
Pyrex Glass double Vessel Assembly with Vessel supporter Top Plate (pH,DO,Anti-foam, Thermo well, Inoculation, Sampling 4poin feeding points). Ports, Blade, Foam Breaker Pyrex Glass Condenser, Ring type Sparger, Removal Baffle Powerful DC Motor, Seal, Pyrex Glass Vessel Silicon Tube, Vessel packing, Cold Finger Heating Plate(Heater, water in/out ports) * Double vessel doesn’t need Heating Plate but Water bath for exclusive use
  • Type               : Double Wall and Dished Bottom type
  • Vessel material  : Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Bowl Vessel

Order No. Vessel Vol. (L)
1-VB-3L
3L
1-VB-5L
5L
1-VB-7L
7L
1-VB-10L
10L
1-VB-14L
14L
1-VB-19L
19L
1-VS-14L
14L
1-VS-19L
19L
Bowl
Pyrex glass single Vessel with BOWL type low plate Top Plate (pH, DO, Anti-foam, Thermo well, Inoculation, Sampling 4 point feeding ports SUS ports Cap, Blade, Foam Breaker Pyrex Glass Condenser, Ring type sparger, Removal Baffle Powerful DC Motor, Single Mechanical Seal, Pyrex Glass Vessel Silicon Tube, Quick coupling, Vessel Packing Heating Plate (Heater, Water in/out ports)
  • Type               : Single Wall and Bowl Bottom
  • Vessel material  : Single well – Borosilicate glass, Bowl – SUS316L
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Sparger
  • Air Condenser   : SUS Condenser (Stainless Steel)
  • Vessel glass packing
  • Vessel port packing
  • Inlet filter           : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter         :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control         : Manual
  • Sampling port       : T-pipe type manual sampler

Small Volume Vessel

Order No. Vessel Vol. (L)
1-VSV-50m
50m
1-VSV-100m
100m
1-VSV-200m
200m
1-VSV-300m
300m
1-VSV-500m
500m
1-VSV-1000m
1000m
Small Vol. Vessel

Single Round Vessel

Order No. Vessel Vol. (L)
1-VSR-500m
500m
1-VSR-1L
1L
1-VSR-2L
2L
1-VSR-3L
3L
1-VSR-5L
5L
1-VSR-7L
7L
1-VSR-10L
10L
1-VSR-14L
14L
1-VSR-19L
19L
Animal cell culture Vessel 3L
Mass cultivation of animal cells has intensively been investigated even thought cell culture techniques have been developed for long times. There have been several difficulties in maintaining large quantities of live cells, especially more than million of cells per m/ in over five liter vessel. Fragility of animal cell, sophisticated culture conditions less economical feasibility of being scaled-up were major bottle necks in idustrializing cell culture technologies to produce a large amount of pharmaceuticals, tPA, interferon, EPO and many other MAb.
  • Type : Single round Vessel
  • Vessel material: Borosilicate glass
  • Top Plate  : SUS 316L
  • pH, DO, Anti-foam, Temp., Inoculation, Sampling, Feeding, Cold Finger, Sparger, Heater Plate
  • Air Condenser : SUS Condenser (Stainless Steel)
  • Vessel glass packing and Vessel port packing
  • Inlet filter : 0.2µm filter interchangeable and repeatedly autoclavable (Sparger line)
  • Outlet filter :0.2µm filter interchangeable and repeatedly autoclavable (Condensor line)
  • Flow control : Manual
  • Sampling port : T-pipe type manual sampler

Lab Scale Plant cell culture Bioreactor BIOEXCEL P : Main Accessories

Mechanically stirred bioreactors

In these bioreactors medium is agitated with the help of a mechanically driven impeller and vary types of impellers have been used. One of these is flat‑blade turbine impeller, in which high agitation breaks incoming air into small bubbles. Mechanically stirred bioreactors depend on impellers, including a helical ribbon impeller, magnetic stirrers, or vibrating perforated plates.

Pneumatically Agitated Bioreactor : Bubble column and Air lift

These are tall and thin in comparison with agitation bioreactors. In the bubble columns air is bubbled at the base of the column, thus medium is agitated. In air lift bioreactors, gas is sparged from the riser section to the top of the column and the medium flows downward in the down corner section. These two sections may be separated using a baffle, a concentric cylinder or an external loop. Circulation in the air lift bioreactor promotes a better mixing and therefore offers advantages in uniformly suspending cells and clumps, although the oxygen transfer rate is low in the down corner section. So the performance of an air lift bioreactors is strongly dependent upon the geometry of the system. Mixing by gas sparging in bubble column or airlift bioreactors lacking impellers or blades is far less damaging for clusters than mechanical stirring, since they were shown to have a lower shearing stress.  The main advantage of airlift bioreactors is their relatively simple construction, the lack of regions of high shear, reasonably high mass and heat transfer and relatively high yields at low input rates.


Lab Scale Bioreactor Controller BIOEXCEL M : Detail function of Operation Program on Bioreactor Controller

DO Control Mode
  • Cascade Select
  • RPM Control Setup, Gas Control Setup, Air Flow Control Setup,
  • RPM, Gas, Air Flow,
DO Control Mode : RPM Setup
  • Action Delay Time, Maximum RPM, Increase Step, Increase Time
  • Stable RPM Return
  • RPM After Gas Insert
DO Control Mode : Gas Setup
  • O2 Gas On/Off Time setup, Use/Not Use
  • N2 Gas On/Off Time setup, Use/Not Use
  • CO2 Gas On/Off Time setup, Use/Not Use
DO Control Mode
DO Control-RPM Setup
DO Control-Gas Setup
pH stat fed Batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, pH Control Use/Not Use
DO stat fed batch
  • Set Point, Active Level (High/Low), Delay Time, Feed Pump On/Off Time, DO Control Use/Not Use
Fed Batch Setup
Fed Batch Setup(pH Stats)060710
Fed Batch Setup(DO Stats)

Calibration Menu

  • –  pH Calibration, DO Calibration, Temperature Calibration,
  • –  Pump Calibration, Gas Calibration, Pressure Calibration

pH Calibration

  • 4 pH, 7 pH, 10 pH, Measuring pH, Temperature and mV
  • Temperature Compensation, Auto Calibration, Auto Calibration Processing Status
  • pH Calibration Status        : Use Buffer, Slope, Run Calibration Status, Last Calibration Date,
  • pH Recalibration               : Process Value, Recalibration Set Value
  • pH Calibration End            : Notice, 1 Point Calibration/pH is Not Calibration

DO Calibration

  • Zero, Sat
  • Temperature Compensation, Auto Calibration
  • DO Calibration Status        : Last Calibration Date, Zero mV, Calibration Temp., Span mV, Max. DO

Temperature Calibration

  • Present Value, Set Temperature Input

Pump Calibration

  • Acid Pump Calibration       : Active Time Input, Input Volume(㎖), Pump On Start
  • Base Pump Calibration      : Active Time Input, Input Volume(㎖), Pump On Start
  • Foam Pump Calibration     : Active Time Input, Input Volume(㎖), Pump On Start
  • Feed Pump Calibration      : Active Time Input, Input Volume(㎖), Pump On Start

Variable Speed Pump Calibration

  • Tube Width, RPM Set, Flow Rate
  • Time Control Use : On/Off Time, Test
pH Calibration2
DO Calibration2
Temperature Calibration2
ORP Calibration Screen
Pump Calibration Menu
Setup Menu
  • pH Setup, DO Setup, Temperature Setup, RPM Setup, Foam Setup, Feed Setup, Alarm Setup
  • pH Setup : pH Set Value
  • Pump Set : Acid On/Off Time, Base On/Off Time
  • DO Setup : DO Set Value Input
  • Temperature Setup : Temperature Set Value Input
  • RPM Setup : Agitation Set Value Input
  • Foam Setup : Foam Set Value Input
  • Pump Set : Pump On/Off Time
  • Feed Setup : Pump Set On/Off Time
Alarm Setup
  • pH Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Acid Alarm Use : Set value(㎖)
  • Base Alarm Use : Set value(㎖)
  • Foam Alarm Use : Set value(㎖)
  • Feed Alarm Use : Set value(㎖)
  • DO Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • Temperature Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
  • RPM Alarm : Low set value, High set value, [Notice : Screen, BUZ, Lamp]
DO Control-Gas Setup
pH Control-Gas Setup
pH Setup
Setup Menu
Feed Setup
Foam Setup
Alarm Setup

Data Log

  • Data Log Setup, Report View, Real Time Graph Trend

Data Log Setup

  • Data Log Destination : PC, Flash Memory, Recorder, Printer
  • PC                                   : Data Log Time Interval, User Name or Description
  • Data Log Item              : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time
  • Flash Memory              : Data Log Time Interval, User Name or Description
  • Data Log Item              : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Recorder

  • Data Log Time Interval, User Name or Description
  • Data Log Item                   : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Printer

  • Data Log Time Interval, User Name or Description
  • Data Log Item  : pH, DO, Temperature, RPM, Pressure, ACID, BASE, FEED, FOAM Detect, Set Value Display, Number, Date & Time

Locking Warning   : Login ID/Pass Word

DataLogMenu
DataLogSetup
BIOEXCEL M Graph Screen 1

System

  • Diagnostic, System Configuration, Advanced User Configuration

Diagnostic

Communication Test(Protocol Test), Temperature Test, Pump Test(Variable/Constant), H/W Port Test, Motor Test(Inverter/PWM), Flash Test, Inverter selection

System Test

Communication Test
  • Status Display, Receipt Information Display, 1 Packet Submit, 1 Packet Receipt, ACK Code, NACK Code, Automatic Submit and Receipt, ID
Temperature Test
  • PID Control Setting, Temperature control constant adjustment, Graphic Checking Display
H/W Port Test
  • Pump                 : Acid, Base, Feed, Foam, Gas1, Gas2, On/Off Checking
  • Temperature    : Heater, Cooler, On/Off Checking
  • Motor                 : Set Value, RPM Read Checking
System Configuration
  • pH Control Configuration, Temperature Control & Sensor Configuration, Anti Foam Configuration, DO Sensor & Gas Configuration, Motor Select Configuration, Pump(Kind) Configuration, Communication Configuration, Touch Calibration, Net Work Configuration, Control Board Configuration, Protocol Configuration, Remote Control Configuration, H/W Module, Use/Not Use
Advanced User Configuration
  • pH Configuration, DO Configuration, Temperature Configuration, Other
pH Configuration
Temp Control&Sensor Configuration
Temp Control&Sensor Configuration(060913)
System
  • Diagnostic, System Configuration, Advanced User Configuration
Diagnostic
  • Communication Test(Protocol Test), Temperature Test, Pump Test(Variable/Constant), H/W Port Test, Motor Test(Inverter/PWM), Flash Test, Inverter selection
온도테스트
System Configuration
Advanced User Configuration
Advanced User Configuration
  • pH Configuration, DO Configuration, Temperature Configuration, Other
Advanced User DO Configuration
Advanced User Configuration
Advanced User Configuration other(060613)
Advanced User Configuration Agitation(061221)
User Feeding Control Select
User Feeding Pump Control
User Feeding Gas Control

BIOEXCEL M Program on Control Panel


Calibration Menu

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Control Menu

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Setup Menu

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SYSTEM MENU : Advanced User
System Config
System Diagonostic
Progrma Map of BIOEXCEL M Bioreactor and Fermentor Controller
controller 화면 한글설명
Main Menu 설명
Setup Mene 설명
Calibration Menu 설명
System Menu 설명
Advanced User Configuration 설명
Data Log Menu

DataLogMenu
Data Log Setup

DataLogSetup
Graph Screen

BIOEXCEL M Graph Screen 2

Fermentation Culture Method

Batch Culture
Batch Culture
A closed system initiated by the inoculation of microorganisms under certain environmental conditions such as specific nutrients, temperature, pressure, aeration and so on. Only few generations of microorganisms can grow before given nutrients are vanished.
  1. Closed system : only open to air
  2. Surface culture, submerged culture
  3. Parameter monitor & control
  4. Biomass growing up
Fed-Batch Culture

Fed-Batch

One or more nutrients (substrates) are fed to the bioreactor during cultivation and in which the product or cultured broth remains in the bioreactor until the end of the run.

  1. Open system
  2. High cell density culture
  3. Extended cultivation time
  4. Increase cell efficiency
Continuous Culture

Continuous culture

Cell culture method which keeps culture volume constant. Fresh medium flows into bioreactor continuously and part of the medium in the reactor is withdrawn from the bioreactor at the same flow rate of the inlet flow

  1. Chemostat : constant chemical environment characteristic of the steady state
  2. Control the growth rate with feeding rate
  3. Strain selection: faster growth
  4. Contamination : strict maintenance
  5. Cell physiology research
Fresh growth medium is added continuously during fermentation and spent medium is removed. (or cells itself are removed)